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Revisión actual del 12:52 6 nov 2019
For HN treatment, the nutrient resolution consisted of (mM): . mM Ca(NO), . KSO, . MgSO, . KCl, . KHPO, HBO, MnSO, CuSO, ZnSO, (NH)MoO, . FeEDTA, withTable . Summary from the investigated traits within this study along with the measurementsClassificationNUErelated traitsTraitGrain yield Natural Product Library CAS nitrogen use efficiency Nitrogen uptake Nitrogen uptake efficiency Nitrogen utilization efficiency Stover yield Harvest index Grain nitrogen concentration Stover nitrogen concentration Nitrogen harvest index The amount of seminal roots The amount of crown roots The amount of lateral rootsAbbreviationsGY NUE Nup NupE NutE SY HI GNC SNC NHI SRN CRN LRNUnitsgm gg g m gg gg g m gg g m g m number quantity numberTrait measurements Grain yieldtotal level of nitrogen Human IgG1 Control Protocol provide GY GNC + SY SNC Total N uptaketotal amount of nitrogen provide Grain yieldtotal nitrogen content material GY(GY + SY) Kjeldahl process Kjeldahl technique GY GNC (GY GNC + SY SNC) Typical variety of the two plants Typical quantity of the two plants Count number of the lateral root within cm from the very first emerged lateral root within the main root. Measured with a ruler Measured with a ruler Measured using a ruler Dried and weighted using a balance (g) Dried and weighted using a balance (g) Root dry weightshoot dry weightRSArelated traitsThe length of main roots The length of seminal roots The length of crown roots Root dry weight Shoot dry weight Root to shoot ratioPRL SRL CRL RDW SDW RScm cm cm mg plant mg plant mgmgLi et al.pH For LN therapy, the . mM Ca(NO) was replaced by . mM Ca(NO) plus . mM CaCl within the initial week, and was then substituted by . mM CaCl till the harvest. The nutrient solution was renewed just about every days and aerated by a pump. The maize seedlings had been grown in a growth chamber with controlled situations: throughout ah lightdark cycle, having a light density ofmol ms that was measured at canopy height. The hydroponically grown two parent lines andRILs had been evaluated by 3 independent experiments (E). Each experiment was performed in a absolutely randomized style with 3 replicates for two N therapies. The value of each replicate was represented by the implies of two seedlings for each RIL or six seedlings for every parental line. The seedlings have been harvested at the developmental stage with five to six visible leaves (days right after seed germination), along with the plants grown below LN condition showed common Ndeficiency symptoms. Roots have been separated in the shoot and stored at ahead of the measurements as described in Table.The phenotypic correlation and principal component evaluation (PCA). Because the same QTLs for GY and NUE, and for Nup and NupE, had been also expected, GY and Nup traits have been omitted from the subsequent QTL analysis. Hydroponics experiments Maize seeds were sterilized formin in a remedy of HO, washed with distilled water, soaked in saturated CaSO forh, and then germinated within the dark on moist filter paper at space temperature. Afterdays the germinated seeds were wrapped in a moist filter paper roll and grown until the stage of two visible leaves. The uniform seedlings were then selected and transferred into a plastic tub (cm, length width height) containingL nutrient answer. The seminal root Bioactive Compound Library In Vitro numbers (SRN) and crown root numbers (CRN) were counted.